Pdgfra-creer

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August undefined, 2024

Splet12. sep. 2024 · Pdgfra inactivation was induced by tamoxifen (TM) in CAGG-CreER;Pdgfra flox/flox mice to obtain mice with global inactivation of Pdgfra (CAGG-iKO mice; a—i). The CAGG-iKO mice with OPC repopulation were defined as “chronic” CAGG-iKO (termed C-KO mice in this study). Identically treated Pdgfra flox/flox mice (Flox mice) were used as ... Splet27. jul. 2024 · Thus, the PDGFRβ-P2A-CreER T2 sequence in the construct allows for production of both PDGFRβ and CreER T2 at similar expression levels via ribosome …

Bone-derived sclerostin and Wnt/β-catenin signaling regulate …

Splet31. okt. 2024 · PDGFRα signaling does not regulate the generation and differentiation of cortical OPCs. Fate-mapping studies in the PDGFRα CreER; Sox10-GFP/tdTom double-transgenic mice of either sex have further corroborated the PDGFRα-independent oligodendrocyte lineage. This study provides additional missing genetic evidence for … Splet02. feb. 2024 · We developed a dual-recombinase intersectional targeting approach by using Pdgfra-DreER, Pdgfrb-CreER, and a Cre/Dre double-dependent reporter Ai66 to mark … gerber organic lil crunchies

PDGFRb+ mesenchymal cells, but not NG2+ mural cells, contribute …

Splet032770 B6.129S- Pdgfra tm1.1(cre/ERT2)Blh /J Pdgfrα-CreER T2 mice have a cre/ERT2 fusion gene inserted into the start codon of the platelet derived growth factor receptor, … Splet02. feb. 2024 · To test whether periblasts that are PDGFRa + PDGFRb + were labeled by tdTomato after tamoxifen treatment, we administered the Pdgfra-DreER;Pdgfrb-CreER;Ai66 pups with tamoxifen at P14 and collected the hearts 48 h later. tdTomato signals were detected in the hearts of Pdgfra-DreER;Pdgfrb-CreER;Ai66 but not in the hearts of … SpletAssigned HPA protein class (es) for the encoded protein (s). Number of protein-coding transcripts from the gene as defined by Ensembl. A summary of the overall protein … christina\u0027s frisco

Generation and characterization of PDGFRα-GFPCreERT2 knock-In …

Sweet Dre-ams about intersectional genetics: Cell Stem Cell

SpletWe have generated a novel mouse line expressing tamoxifen-inducible Cre-recombinase under the control of the platelet-derived growth factor receptor β promoter: PDGFRβ-P2A … SpletSingle-cell RNA-sequencing was used to characterize distinct vessel-forming populations and their interactions. Results: Two populations of distinct vascular stem/progenitor cells (VSPCs) were identified from adipose-derived mesenchymal stromal cells: VSPC1 is CD45-Ter119-Tie2+PDGFRa-CD31+CD105 high Sca1 low, which gives rise to stunted vessels ... gerber organic oatmeal cereal walmartSplet08. okt. 2008 · By in situ hybridization, the authors found that Pdgfra mRNA was detected in nearly all cre mRNA–expressing cells in both gray and white matter. A tradeoff with inducible control is that the... gerber organic oatmeal cereal arsenic

"Splet25. jan. 2024 · The PDGFRα-CreER TM animals were a kind gift from the Dwight Bergles laboratory 56 and are commercially available (The Jackson Laboratory, Stock No. … " - Pdgfra-creer

Pdgfra-creer

Splet25. jan. 2024 · Platelet-derived growth factor receptor α (PDGFRα) is an emerging stem cell marker and can identify progenitor cell populations in multiple tissues, such as the … Splet31. okt. 2024 · PDGFRα, specifically expressed by immature oligodendrocyte progenitor cells (OPCs) in the CNS, plays a critical role in OPC proliferation and migration. However, …

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Splet10. apr. 2024 · For Pdgfra DreER-tdTomato, Col1a2 CreER Tnc fl/fl and Tnc fl/fl mice, tamoxifen was administered at 5-week-old for 5 consecutive days, and psoriasis mouse model was performed after 2-week washout ... Splet28. avg. 2016 · To confirm specificity of PDGFRa-CreER and exclude the possibility of non-specific Cre activation with CTX injury, we compared tamoxifen and vehicle-treated PDGFRa-CreER mice harbouring the mT/mG loxP flanked conditional reporter. In these studies, GFP+ cells, indicative of Cre-mediated recombination, were observed only in …

http://genesdev.cshlp.org/content/suppl/2016/11/02/gad.285429.116.DC1/Supplemental_Text_Legends.docx Splet19. nov. 2024 · Pdgfra-CreER T2 and mT/mG mice were crossed to generate Pdgfra-CreERT2; mT/mG reporter mice, which were used for all experiments as previously described. 26, 27 Tamoxifen (TM; product number...

SpletIt can be classified into cells positive for vascular endothelial growth factor receptor 2 (VEGFR2 or FLK1) or platelet-derived growth factor receptor α (PDGFRα), representing lateral and paraxial mesoderm, respectively. Era and colleagues generated knock-in mice carrying GFP-Cre-ERT2 at the start codon of Pdgfra [1]. They confirmed Cre ... Splet01. dec. 2010 · The adult mammalian brain and spinal cord contain glial precursors that express platelet-derived growth factor receptor α subunit (PDGFRA) and the NG2 proteoglycan. These "NG2 cells" descend from oligodendrocyte precursors in the perinatal CNS and continue to generate myelinating oligodendrocytes in the gray and white matter …

Somatic mutations that cause the fusion of the PDGFRA gene with certain other genes occur in hematopoietic stem cells and cause a hematological malignancy in the clonal hypereosinophilia class of malignancies. These mutations create fused genes which encode chimeric proteins that possess continuously active PDGFRA-derived tyrosine kinase. They thereby continuously stimulate cell growth and proliferation and lead to the development of leukemias, lymphomas, and myelody…

SpletWe developed a dual-recombinase intersectional targeting approach by using Pdgfra-DreER, Pdgfrb-CreER, and a Cre/Dre double-dependent reporter Ai66 to mark PDGFRa + PDGFRb + periendothelial fibroblasts (periblasts) to study their contribution to CAs. gerber organic fruit infused water gerber organic pear spinachSplet28. avg. 2016 · To confirm specificity of PDGFRa‐CreER and exclude the possibility of non‐specific Cre activation with CTX injury, we compared tamoxifen and vehicle‐treated PDGFRa‐CreER mice harbouring the mT/mG loxP flanked conditional reporter. In these studies, GFP+ cells, indicative of Cre‐mediated recombination, were observed only in … gerber organic pouches couponSplet15. sep. 2011 · This PDGFR-CreER mouse model is a useful resource for studying cell specific genetic regulation of oligodendrocytes in vivo. Value Proposition: Oligodendrocytes and pericytes in the central nervous system (CNS) express NG-2 and PDGFR. NG-2 promoters are commonly used to label NG2+ cells in transgenic models, but using a … gerber organic pouchesSplet21. apr. 2024 · In the present study, we characterized a Pdgfra-CreER knock-in line and examined PDGFRa-derived cells during homeostasis and injury response in large vessels … We would like to show you a description here but the site won’t allow us. gerber organic peasSplet08. okt. 2008 · Platelet-derived growth factor α receptor (PDGFRA)/NG2–expressing glia are distributed throughout the adult CNS. They are descended from oligodendrocyte … christina\\u0027s gift boxSplet03. jun. 2024 · For this, they created Pdgfra-DreER red fluorescent reporter mice and crossed them with Pdgfrb-CreER green fluorescent reporter mice. They used this tamoxifen-inducible system to screen adult organs for three populations of labeled cells: Pdgfra+ (red), Pdgfrb+ (green), and Pdgfra+Pdgfrb+ (yellow). christina\u0027s ghost book