WebMar 3, 2024 · So should be important for high NA, even if the illumination is just a thin ring. At NA 1.4 and 1.5 the angles are so shallow, aberrations likely get gigantic. So the condenser needs to be well made and relatively sharp, like a high NA objective - with its shallow focus depth, hence tight slide thickness. WebOct 21, 2014 · A condenser iris controls the NA of the transmitted image as well as the brightness and contrast. There is a high NA condenser lens (0.69) (Thorlabs, part# C330TME-B) that enables a crisp transmitted image of the sample down to fields of view of approximately 100 microns.

Nikon Condensers - University of Arizona

WebUncoated Aspheric Condenser Lenses. Our uncoated aspheric condenser lenses are fabricated out of Schott B 270® Ultra-White or equivalent glass. These condenser lenses provide excellent transmission in the visible to near-infrared regions. The aspheric condenser lens surface is molded, while the plano or spherical surface is ground and … fixd pros and cons

Understanding the microscope. 6. Condensers and contrast

Webusing a high NA dark field condenser. High NA dark field condensers have a fixed focal length at which they optimally work. The manufacturer may recommend a slide thickness or this value may be printed on the condenser. A standard slide is 1.2 mm thick, but slides, even from the same box, vary in thickness. The slide should be no more than ± WebFirst, place a stained specimen on the stage and rotate the nosepiece until the objective (preferably a low power 10x or 20x) is in place. Switch on the light source. Open all … WebThus in case of a closed system, mixing high pressure and low pressure condensate increases the back pressure on the lowest pressure header, reducing effective … fix downspout